A Conformational Study of Yeast Phenylalanine Transfer Ribonucleic Acid
نویسندگان
چکیده
منابع مشابه
A conformational study of yeast phenylalanine transfer ribonucleic acid.
Yeast phenylalanine tRNA (tRNAPhe) exhibits a 4$-fold enhancement of fluorescence in response to the addition of Mg2+. Concurrently, over the same concentration range (0 to 0.4 m&r) of Mg2+, the circular dichroism peak at 263 nm displays a 20% increase in ellipticity and a blue shift of 2 nm. The existence of the negative circular dichroism band at 295 nm appears to be cation-dependent. This co...
متن کاملA Conforrnational Study of Yeast Phenylalanine Transfer Ribonucleic Acid*
Yeast phenylalanine tRNA (tRNAPhe) exhibits a 4$-fold enhancement of fluorescence in response to the addition of Mg2+. Concurrently, over the same concentration range (0 to 0.4 m&r) of Mg2+, the circular dichroism peak at 263 nm displays a 20% increase in ellipticity and a blue shift of 2 nm. The existence of the negative circular dichroism band at 295 nm appears to be cation-dependent. This co...
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Yeast tRNAPhe has been cross-linked photochemically to three aminoacyl-tRNA synthetases, yeast phenylalanyltRNA synthetase, Escherichia coli isoleucyl-tRNA synthetase, and E. coli valyl-tRNA synthetase. The two non-cognate enzymes are known to interact with tRNAPhe. In each complex, three regions on the tRNA are found to cross-link. Two of these are common to all of the complexes, while the thi...
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tertiary structure of the molecule. These seem to be in the region of the ‘hinge’ between dihydrouridine and the ribosylthymine-pseudouridine loop. Further, the n.m.r. studies allow one to propose a detailed structural model for the anticodon loop (Ts’o et al., 1975). The problem of the specificity of aminoacylation has been approached by equilibrium and kinetic studies. A striking feature of t...
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We are attempting to determine the nature of specific RNA-protein interactions by study of tRNA charging by homologous and heterologous enzymes. tRNAklatRNARNA3 t were prepared by phenoxyacetylation of alanyl-tRNA and separation on benzoylated DEAE-cellulose (Gillam, Blew, Warrington, von Tigerstrom & Tener, 1968). A partially purified alanyl-tRNA synthetase from yeast forms alanyl-tRNAAla tand...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62539-6